Journal: Cell

Article Title: Vaccine protection against the SARS-CoV-2 Omicron variant in macaques

doi: 10.1016/j.cell.2022.03.024

Figure Lengend Snippet: Humoral immune responses following vaccination Antibody responses at weeks 0 (baseline), 8 (post-prime), 14 (pre-boost), and 18 (post-boost) following vaccination with BNTx3, BNTx2/Ad26, Ad26/BNT, Ad26x2, or sham (N = 30; N = 6/group). (A) Neutralizing antibody (NAb) titers by a luciferase-based pseudovirus neutralization assay. (B) Receptor-binding domain (RBD)-specific binding antibody titers by ELISA. Responses were measured against the SARS-CoV-2 WA1/2020 (black), B.1.617.2 (Delta; blue), B.1.351 (Beta; red), and B.1.1.529 (Omicron; green) variants. Dotted lines represent limits of quantitation. Medians (red bars) are shown. Omicron-specific NAbs in the vaccinated groups were compared with the sham controls by two-sided Mann-Whitney tests. ∗ p < 0.05.

Article Snippet: SARS-CoV-2 (Omicron) RBD , Sino Biological , Cat # 40592-V08H121.

Techniques: Luciferase, Neutralization, Binding Assay, Enzyme-linked Immunosorbent Assay, Quantitation Assay, MANN-WHITNEY

Journal: Cell

Article Title: Vaccine protection against the SARS-CoV-2 Omicron variant in macaques

doi: 10.1016/j.cell.2022.03.024

Figure Lengend Snippet: Cellular immune responses following vaccination T cell responses at weeks 14 (pre-boost) and 18 (post-boost) following vaccination with BNTx3, BNTx2/Ad26, Ad26/BNT, Ad26x2, or sham (N = 30; N = 6/group). (A and B) Pooled peptide spike-specific IFN-γ (A) CD8+ T cell responses and (B) CD4+ T cell responses by intracellular cytokine staining assays. Responses were measured against the SARS-CoV-2 WA1/2020 (black), B.1.617.2 (Delta; blue), and B.1.1.529 (Omicron; green) variants. Dotted lines represent limits of quantitation. Medians (red bars) are shown. Omicron-specific CD8+ and CD4+ T cell responses in the vaccinated groups were compared with the sham controls by two-sided Mann-Whitney tests. ∗ p < 0.05.

Article Snippet: SARS-CoV-2 (Omicron) RBD , Sino Biological , Cat # 40592-V08H121.

Techniques: Staining, Quantitation Assay, MANN-WHITNEY

Journal: Cell

Article Title: Vaccine protection against the SARS-CoV-2 Omicron variant in macaques

doi: 10.1016/j.cell.2022.03.024

Figure Lengend Snippet: Viral loads following SARS-CoV-2 Omicron challenge (A) Log subgenomic RNA (sgRNA) copies/mL in bronchoalveolar lavage (BAL) following SARS-CoV-2 Omicron challenge. (B) Log subgenomic RNA (sgRNA) copies/swab in nasal swabs (NS) following SARS-CoV-2 Omicron challenge. Medians (red lines) are shown.

Article Snippet: SARS-CoV-2 (Omicron) RBD , Sino Biological , Cat # 40592-V08H121.

Techniques:

Journal: Cell

Article Title: Vaccine protection against the SARS-CoV-2 Omicron variant in macaques

doi: 10.1016/j.cell.2022.03.024

Figure Lengend Snippet: Comparison of peak and day 4 viral loads (A) Log subgenomic RNA (sgRNA) copies/mL in bronchoalveolar lavage (BAL) at peak and on day 4 following SARS-CoV-2 Omicron challenge. (B) Log subgenomic RNA (sgRNA) copies/swab in nasal swabs (NS) at peak and on day 4 following SARS-CoV-2 Omicron challenge. Dotted lines represent limits of quantitation. Medians (red bars) are shown. Vaccinated groups were compared with the sham controls by two-sided Mann-Whitney tests. ∗ p < 0.05.

Article Snippet: SARS-CoV-2 (Omicron) RBD , Sino Biological , Cat # 40592-V08H121.

Techniques: Quantitation Assay, MANN-WHITNEY

Journal: Cell

Article Title: Vaccine protection against the SARS-CoV-2 Omicron variant in macaques

doi: 10.1016/j.cell.2022.03.024

Figure Lengend Snippet: TCID50 titers, related to Figure 3 Log TCID50/mL in bronchoalveolar lavage (BAL) and nasal swabs on day 2 following SARS-CoV-2 Omicron challenge (top). Log TCID50/mL is also shown in nasal swabs on day 7 following SARS-CoV-2 Omicron challenge in the 4 vaccinated animals in the BNTx3 and BNTx2/Ad26 groups and in the 6 sham controls with persistently positive sgRNA levels on day 7 (bottom). Dotted lines represent limits of quantitation. Medians (red bars) are shown. Vaccinated groups were compared with the sham controls by two-sided Mann-Whitney tests. ∗ p < 0.05.

Article Snippet: SARS-CoV-2 (Omicron) RBD , Sino Biological , Cat # 40592-V08H121.

Techniques: Quantitation Assay, MANN-WHITNEY

Journal: Cell

Article Title: Vaccine protection against the SARS-CoV-2 Omicron variant in macaques

doi: 10.1016/j.cell.2022.03.024

Figure Lengend Snippet: Histopathology and immunohistochemistry of Omicron infection, related to Figure 1 (A–K) (A–C) Pharynx and (D–K) lungs from macaques on day 2 following Omicron infection demonstrated lymphoid hyperplasia of the pharynx (A and B), SARS-N positive ciliated epithelial cells in the pharynx (C), foamy macrophages and degenerating neutrophils in bronchiole lumen (D), cellular necrotic debris adhering to bronchiolar ciliated epithelium (E), alveolar syncytia (F), SARS-N-positive ciliated epithelial cells in the pulmonary interstitium (G), neutrophilic bronchitis (H), hyaline membranes (I), endothelialitis (J), and type II pneumocyte hyperplasia (K). Scoring involved assessment of the following lesions: interstitial inflammation and septal thickening, interstitial infiltrate (eosinophils), interstitial infiltrate (neutrophils), hyaline membranes, interstitial fibrosis, alveolar infiltrate (macrophages), bronchoalveolar infiltrate (neutrophils), epithelial syncytia, type II pneumocyte hyperplasia, bronchi infiltrate (macrophages), bronchi infiltrate (neutrophils), bronchi (hyperplasia of bronchus-associated lymphoid tissue), bronchiolar or peribronchiolar infiltrate (mononuclear cells), perivascular infiltrate (mononuclear cells), and endothelialitis. Each feature assessed was assigned a score of: 0, no substantial findings; 1, minimal; 2, mild; 3, moderate; 4, moderate to severe; 5, marked or severe. Scores were added for all lesions across all lung lobes for each macaque, for a maximum possible score of 600 for each macaque. (L) Summary of lung pathology scores from SARS-CoV-2 WA1/2020- and Omicron-infected macaques. Medians (red bars) are shown. Dotted line represents no pathology. Lung pathology scores were compared in macaques infected with Omicron versus WA1/2020 by two-sided Mann-Whitney tests. ∗ p < 0.05.

Article Snippet: SARS-CoV-2 (Omicron) RBD , Sino Biological , Cat # 40592-V08H121.

Techniques: Histopathology, Immunohistochemistry, Infection, MANN-WHITNEY

Journal: Cell

Article Title: Vaccine protection against the SARS-CoV-2 Omicron variant in macaques

doi: 10.1016/j.cell.2022.03.024

Figure Lengend Snippet:

Article Snippet: SARS-CoV-2 (Omicron) RBD , Sino Biological , Cat # 40592-V08H121.

Techniques: Infection, Recombinant, Binding Assay, Blocking Assay, Software

Journal: Cell Host & Microbe

Article Title: 35B5 antibody potently neutralizes SARS-CoV-2 Omicron by disrupting the N-glycan switch via a conserved spike epitope

doi: 10.1016/j.chom.2022.03.035

Figure Lengend Snippet: Neutralizing activity of 35B5 against SARS-CoV-2 variants (A) Binding capacities and neutralizing activities of 35B5 against the S proteins of the SARS-CoV-2 WT and the Delta and Omicron variants. (B) Representative negative-staining EM micrographs of the Omicron S-ECD trimer after the treatment of 35B5. The Omicron S-ECD (the stable S-HexaPro mutant) trimer proteins were treated with or without 35B5 for 3 min at 4°C before negative-staining analysis. Scale bar, 50 nm. (C) Binding capacities and neutralizing activities of 35B5 against the pseudoviruses of the SARS-CoV-2 WT and the Delta and Omicron variants. (D) Neutralizing activity of 35B5 against the authentic SARS-CoV-2 Omicron. Data in (A), (C), and (D) are representative of one independent experiment out of two or three. Error bars in (A), (C), and (D) indicate the SEM.

Article Snippet: The next day, mAbs were serially diluted in complete media, mixed with WT pseudoviruses (Sino Biological, PSV001) or Delta pseudoviruses (Sino Biological, PSV011) or Omicron pseudoviruses (Sino Biological, PSV016) and incubated for 1 h at 37°C.

Techniques: Activity Assay, Binding Assay, Negative Staining, Mutagenesis

Journal: Cell Host & Microbe

Article Title: 35B5 antibody potently neutralizes SARS-CoV-2 Omicron by disrupting the N-glycan switch via a conserved spike epitope

doi: 10.1016/j.chom.2022.03.035

Figure Lengend Snippet:

Article Snippet: The next day, mAbs were serially diluted in complete media, mixed with WT pseudoviruses (Sino Biological, PSV001) or Delta pseudoviruses (Sino Biological, PSV011) or Omicron pseudoviruses (Sino Biological, PSV016) and incubated for 1 h at 37°C.

Techniques: Expressing, Reporter Assay, Recombinant, Variant Assay, Mutagenesis, Software

Journal: medRxiv

Article Title: Systems biological assessment of the temporal dynamics of immunity to a viral infection in the first weeks and months of life

doi: 10.1101/2023.01.28.23285133

Figure Lengend Snippet: Memory B and T cell response, related to A) Gating strategy used for SARS-CoV-2 spike specific IgG+ memory B cell staining and single-cell sorting. Gating was on singlets that were CD20+ and CD3-CD14-IgM-IgD-CD27low/+ IgG+. Sorted cells were Wuhan spike-AlexaFluor 488+ and/or Omicron spike-BV421+. B) The percentage of SARS-CoV-2 spike-specific IgG+ memory B cells in healthy, acute, and convalescent infant individuals. The sample number for each group is indicated in brackets. C) As in (B), the percentage of SARS-CoV-2 spike-specific IgG+ memory B cells in adult individuals with mild, severe, and ICU symptoms and in adult convalescent individuals. The sample number for each group is indicated in brackets. D-F) T cells were stimulated with overlapping peptides against WT (D-F) and Omicron (F) variants. Cytokine production was determined via flow cytometry. D) Box plot showing the fraction of responding T cells at different infection stages. E) Box plot showing the fraction of multifunctional T cells at different infection stages. F) Comparison of the multifunctional T cell response after stimulation with WT and Omicron (Om) peptides. Statistical comparisons were conducted with Wilcoxon rank sum test. Solid line indicates median healthy response; dashed line indicates 3x median healthy response.

Article Snippet: The following antibodies were used: IgD PE (BD Biosciences, 555779), IgM PerCP-Cy5.5 (BioLegend, 314512), CD20 APC-H7 (BD Biosciences, 560734), CD27 PE-Cy7 (BioLegend, 302838), CD14 PE/Dazzle™ 594 (BioLegend, 301852), CD16 BV605 (BioLegend, 302040), IgG BV650 (BD Biosciences, 740596), CD3 BUV737 (BD Biosciences, 612750) and Alexa Fluor 488-labeled Wuhan spike (SinoBiological, 40589-V27B-B), and BV421 labeled Omicron Spike (SinoBiological™, 40589-V49H3-B).

Techniques: Staining, FACS, Flow Cytometry, Infection